Journal: International Journal of Molecular Sciences

Article Title: Multisite Injections of Canine Glial-Restricted Progenitors Promote Brain Myelination and Extend the Survival of Dysmyelinated Mice

doi: 10.3390/ijms251910580

Figure Lengend Snippet: The phenotype of canine glial-restricted progenitors (cGRPs) in vitro. ( A ) Morphology of cGRPs observed during 14 days in culture. Notice bipolar morphology (arrow) and formation of cellular aggregates (symbol of a star). Abbreviations: BF, bright field; DIV, day in vitro. ( B ) Phenotypic characterization of cGRPs by immunocytochemistry. Glial progenitors/oligodendrocyte precursors were detected by expression of A2B5, NG2, PDGFRα, Olig1, Olig2, O4, and CNPase. Anti-Ki67 antibody was used as a proliferation marker and was detectable in approximately 17% of cultured cells. Anti-MBP and -GalC antibodies were used for the detection of oligodendrocytes. Nuclei were counterstained with DAPI. Scale bar: 100 μm.

Article Snippet: 4 °C: anti-Olig1 (1:500) (AB15620, Merck KGaA, Darmstadt, Germany); anti-Olig2 (1:500) (ABN899, Merck KGaA, Darmstadt, Germany); anti-Ki67 (1:10) (PA0118, Leica Biosystems, Wetzlar, Germany); anti-PDGFR (1:200) (sc-338, Santa Cruz Biotechnology, Dallas, TX, USA); anti-A2B5 (1:200) (MAB312R, Merck KGaA, Darmstadt, Germany); anti-GFAP (1:200) (Z0334, DAKO, Jena, Germany); anti-NG2 (1:200) (AB5320, Merck KGaA, Darmstadt, Germany); anti-CNPase (1:200) (AB9342, Merck KGaA, Darmstadt, Germany); anti-O4 (1:200) (MAB345, Merck KGaA, Darmstadt, Germany); anti-GalC (1:200) (MAB342, Merck KGaA, Darmstadt, Germany); anti-MBP (1:200) (MAB382, Merck KGaA, Darmstadt, Germany).

Techniques: In Vitro, Immunocytochemistry, Expressing, Marker, Cell Culture